Displacement of the previously bound, and quenched, fluorescent indicator (I) by coordination of the pyrophosphate ion to the two CuII centers of the receptor R results in the generation of the natural fluorescence of the indicator, and thus signals pyrophosphate recognition (see scheme). Discrimination from interfering anions (HPO42−, SO42−, NO3−, Cl−, NCO−, N3−, acetate, benzoate) can be achieved by tuning the indicator affinity towards the receptor.

Pyrophosphate Detection in Water by Fluorescence Competition Assays: Inducing Selectivity through the Choice of the Indicator.

FABBRIZZI, LUIGI;TAGLIETTI, ANGELO MARIA
2002-01-01

Abstract

Displacement of the previously bound, and quenched, fluorescent indicator (I) by coordination of the pyrophosphate ion to the two CuII centers of the receptor R results in the generation of the natural fluorescence of the indicator, and thus signals pyrophosphate recognition (see scheme). Discrimination from interfering anions (HPO42−, SO42−, NO3−, Cl−, NCO−, N3−, acetate, benzoate) can be achieved by tuning the indicator affinity towards the receptor.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/116605
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 262
  • ???jsp.display-item.citation.isi??? 256
social impact