Steady-state and pulse-chase collagen analyses are powerful approaches to investigate in vitro the structure of collagen and its kinetic of secretion, respectively. The electrophoretic analysis of purified 3H-proline-labeled collagen allows to determine the nature and post translational modifications of its α chains, whereas short-pulse labeling can be used to follow collagen secretion over time.
Steady-state and pulse-chase analyses of fibrillar collagen
Forlino A.;Tonelli F.;Besio R.
2019-01-01
Abstract
Steady-state and pulse-chase collagen analyses are powerful approaches to investigate in vitro the structure of collagen and its kinetic of secretion, respectively. The electrophoretic analysis of purified 3H-proline-labeled collagen allows to determine the nature and post translational modifications of its α chains, whereas short-pulse labeling can be used to follow collagen secretion over time.File in questo prodotto:
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