Using a PCR based method, we determined hypervariable markers of the Thyroid Peroxidase gene (TPO-VNTR markers) by size classification of alleles in a total of 146 blood samples (76 from Sicily and 70 from Lombardy, South and North Italy respectively). A total of 22 alleles were identified. They varied in size from 6 (0.4 Kbp) to 27 (1.5 Kbp) repeats and showed a unimodal distribution (peak at 15 repeat number). Data were compared with those previously obtained in two other Italian samples from Calabria (South Italy) and from Sardinia. Heterogeneity G tests on allele frequency distributions led to the following conclusions: 1) as observerd for the majority of the known polymorphisms, the Sardinian sample is clearly distinguishable from all the others; 2) although on the whole no significant heterogeneity emerges from the comparison between southern and northern Italians, certain allelic frequencies are able to distinguish between groups.

Hypervariability of the human thyroid peroxidase gene in Italy

SEMINO, ORNELLA;
1995-01-01

Abstract

Using a PCR based method, we determined hypervariable markers of the Thyroid Peroxidase gene (TPO-VNTR markers) by size classification of alleles in a total of 146 blood samples (76 from Sicily and 70 from Lombardy, South and North Italy respectively). A total of 22 alleles were identified. They varied in size from 6 (0.4 Kbp) to 27 (1.5 Kbp) repeats and showed a unimodal distribution (peak at 15 repeat number). Data were compared with those previously obtained in two other Italian samples from Calabria (South Italy) and from Sardinia. Heterogeneity G tests on allele frequency distributions led to the following conclusions: 1) as observerd for the majority of the known polymorphisms, the Sardinian sample is clearly distinguishable from all the others; 2) although on the whole no significant heterogeneity emerges from the comparison between southern and northern Italians, certain allelic frequencies are able to distinguish between groups.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/130966
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact