A pivotal role has been ascribed to oxidative stress in determining the unbalance between protein synthesis and degradation leading to muscle atrophy in many pathologic conditions and in disuse. However, a large variability in disuse induced alteration of redox homeostasis through muscles, models and species emerges from the literature. Whereas the causal role of oxidative stress appears well established in the mechanical ventilation model, findings are less compelling in the hindlimb unloaded mice and very limited in humans. The mere coexistence of muscle atrophy, indirect indexes of increased reactive oxygen species (ROS) production and impairment of antioxidant defence systems, in fact, does not unequivocally support a causal role of oxidative stress in the phenomenon. We hypothesise that in some muscles, models and species only, due to a large redox unbalance, the leading phenomena are activation of proteolysis and massive oxidation of proteins which would become more susceptible to degradation. In other conditions, due to a lower extent and variable time course of ROS production, different ROS dependent, but also independent intracellular pathways might dominate determining variable extent of atrophy and even dispensable protein oxidation. The ROS production and removal are complex and finely tuned phenomena. They are indeed important intracellular signals and redox balance maintains normal muscle homeostasis and can underly either positive or negative adaptations to exercise. A precise approach to determine the levels of ROS in living cells in various conditions appears of paramount importance to define and support such hypotheses.

Redox homeostasis, oxidative stress and disuse muscle atrophy

PELLEGRINO, MARIA ANTONIETTA;BROCCA, LORENZA;BOTTINELLI, ROBERTO
2011-01-01

Abstract

A pivotal role has been ascribed to oxidative stress in determining the unbalance between protein synthesis and degradation leading to muscle atrophy in many pathologic conditions and in disuse. However, a large variability in disuse induced alteration of redox homeostasis through muscles, models and species emerges from the literature. Whereas the causal role of oxidative stress appears well established in the mechanical ventilation model, findings are less compelling in the hindlimb unloaded mice and very limited in humans. The mere coexistence of muscle atrophy, indirect indexes of increased reactive oxygen species (ROS) production and impairment of antioxidant defence systems, in fact, does not unequivocally support a causal role of oxidative stress in the phenomenon. We hypothesise that in some muscles, models and species only, due to a large redox unbalance, the leading phenomena are activation of proteolysis and massive oxidation of proteins which would become more susceptible to degradation. In other conditions, due to a lower extent and variable time course of ROS production, different ROS dependent, but also independent intracellular pathways might dominate determining variable extent of atrophy and even dispensable protein oxidation. The ROS production and removal are complex and finely tuned phenomena. They are indeed important intracellular signals and redox balance maintains normal muscle homeostasis and can underly either positive or negative adaptations to exercise. A precise approach to determine the levels of ROS in living cells in various conditions appears of paramount importance to define and support such hypotheses.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/226547
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