P29 is a metacestode-specific antigen of E. granulosus protoscolices that was expressed and purified as a recombinant antigen, rec P29 (Ben Nouir et al. 2009). recB2t is a truncated version of antigen B2, and has been expressed as gst-fusion protein and subsequently thrombin-digested and affinity-purifed (Hernandez-Gonzales et al., 2008). Both genes showed potential as tool for follow-up studies after surgery/treatment of cystic echinococcosis (CE) in preliminary studies. Post-surgical cavities may be difficult to diagnose even in referral centers for CE, and conventional serology results may be misleading as they may remain positive even when the cavity no longer contains viable cyst material or protoscoleces, as demonstrated by diagnostic aspiration. In this preliminary, retrospective study, we compared the diagnostic performance of recP29, recB2t and (conventional) EgHf in ELISA by testing 18 patients with CE post-surgical cavities (17 in the liver and 1 in the kidney) and 1 patient with a transitional CE3b cyst in the liver, recP29 was most reliable at indicating the status of residual cavity (i.e. negative also in cases in which recB2t and conventional EgHf were positive). Of note, recP29 was negative in the patient with the CE3b cyst, a stage that is biologically active when assessed by Magnetic Resonance Spectroscopy (Hosch 2008) Our results indicate that recP29 and rec B2t deserve further longitudinal studies with larger series pf patients with CE.

Comparison of P29, B2T and EGHF diagnostic performance (ELISA) in patients with residual cavities after surgery for cystic echinococcosis

BRUNETTI, ENRICO;FILICE, CARLO;
2009-01-01

Abstract

P29 is a metacestode-specific antigen of E. granulosus protoscolices that was expressed and purified as a recombinant antigen, rec P29 (Ben Nouir et al. 2009). recB2t is a truncated version of antigen B2, and has been expressed as gst-fusion protein and subsequently thrombin-digested and affinity-purifed (Hernandez-Gonzales et al., 2008). Both genes showed potential as tool for follow-up studies after surgery/treatment of cystic echinococcosis (CE) in preliminary studies. Post-surgical cavities may be difficult to diagnose even in referral centers for CE, and conventional serology results may be misleading as they may remain positive even when the cavity no longer contains viable cyst material or protoscoleces, as demonstrated by diagnostic aspiration. In this preliminary, retrospective study, we compared the diagnostic performance of recP29, recB2t and (conventional) EgHf in ELISA by testing 18 patients with CE post-surgical cavities (17 in the liver and 1 in the kidney) and 1 patient with a transitional CE3b cyst in the liver, recP29 was most reliable at indicating the status of residual cavity (i.e. negative also in cases in which recB2t and conventional EgHf were positive). Of note, recP29 was negative in the patient with the CE3b cyst, a stage that is biologically active when assessed by Magnetic Resonance Spectroscopy (Hosch 2008) Our results indicate that recP29 and rec B2t deserve further longitudinal studies with larger series pf patients with CE.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/256715
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