With the Feulgen-Naphthol Yellow double reaction we dosed, simultaneously on a cell, [1] the DNA nuclear content (as Feulgen positive material), [2] the protein cell content and [3] the Feulgen chromatin area; the degree of condensation is expressed by the ratio [1]/[3]. The evolution of the lymphocytes culture at different times of PHA stimulation was in this way studied with the aim of defining the existence of cell subpopulations pertaining at different phases or subphases of the cycle.

Some parameters for a cell cycle cytotopochemical study.

FORMENTI, DANIELE;PELLICCIARI, CARLO;REDI, CARLO ALBERTO;SCHERINI, ELDA
1981-01-01

Abstract

With the Feulgen-Naphthol Yellow double reaction we dosed, simultaneously on a cell, [1] the DNA nuclear content (as Feulgen positive material), [2] the protein cell content and [3] the Feulgen chromatin area; the degree of condensation is expressed by the ratio [1]/[3]. The evolution of the lymphocytes culture at different times of PHA stimulation was in this way studied with the aim of defining the existence of cell subpopulations pertaining at different phases or subphases of the cycle.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/469471
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