Two distinct extracellular lipases were obtained from Penicillium solitum 194A and Cladosporium cladosporioides 194B, isolated from domestic compost and dairy wastewater, respectively. They were alkaline enzymes of 42 kDa and 30 kDa, respectively. The molecular mass of P. solitum 194A lipase differed from that of the P. solitum enzyme previously reported, indicating the isolation of a novel lipase and proving the capability of penicillia to secrete lipase isoenzymes. The lipase from C. cladosporioides was more active on esters of medium-chain acids, whereas P. solitum enzyme on substrates with longer chains. C. cladosporioides enzyme displayed higher heat stability than that of P. solitum, preserving full activity up to 48°C and showing a T50 (10 min) of 60°C. The different catalytic properties and the good protein stability make the two enzymes suited for biotechnological applications. Moreover, the two fungal strains may constitute a valuable consortium for lipid-rich waste management.
Biochemistry of Lipolytic Enzymes Secreted by Penicillium solitum and Cladosporium cladosporioides
CHINAGLIA, SELENE;CHIARELLI, LAURENT;Maggi M.;RODOLFI, MARINELLA;VALENTINI, GIOVANNA;PICCO, ANNA MARIA
2014-01-01
Abstract
Two distinct extracellular lipases were obtained from Penicillium solitum 194A and Cladosporium cladosporioides 194B, isolated from domestic compost and dairy wastewater, respectively. They were alkaline enzymes of 42 kDa and 30 kDa, respectively. The molecular mass of P. solitum 194A lipase differed from that of the P. solitum enzyme previously reported, indicating the isolation of a novel lipase and proving the capability of penicillia to secrete lipase isoenzymes. The lipase from C. cladosporioides was more active on esters of medium-chain acids, whereas P. solitum enzyme on substrates with longer chains. C. cladosporioides enzyme displayed higher heat stability than that of P. solitum, preserving full activity up to 48°C and showing a T50 (10 min) of 60°C. The different catalytic properties and the good protein stability make the two enzymes suited for biotechnological applications. Moreover, the two fungal strains may constitute a valuable consortium for lipid-rich waste management.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
