In the last decade the need for portable Surface Plasmon Resonance (SPR) biosensors capable of on-site simultaneous multiple assays increased steadily. Several devices are available affected, however, by limitations in terms of costs, size, complexity and portability. A compact low-cost SPRi biosensor based on a novel method for multi-analyte detection is presented. The prototype consists of a nanohole array biochip integrated with a compact optics and an elaboration system. A CMOS image sensor captures reflected light from the biochip surface irradiated by a 830 nm LED. The entire system is managed by an ARM9 processor. The biosensor was able to detect a ~10-5 RIU change in the refractive index without analyte receptors at a glycerol concentration equal to 0.2%. Results are available in 14 seconds on LCD display and immediately stored to external SD memory. Preliminary experiments confirmed the strong biosensor's usability in a wide range of applications and fields
A Novel Portable Surface Plasmon Resonance Based Imaging Instrument for On-Site Multi-Analyte Detection
RAMPAZZI, SARA;LEPORATI, FRANCESCO;DANESE, GIOVANNI;NAZZICARI, NELSON DAVIDE;FORNASARI, LUCIA;MARABELLI, FRANCO;VALSESIA, ANDREA
2013-01-01
Abstract
In the last decade the need for portable Surface Plasmon Resonance (SPR) biosensors capable of on-site simultaneous multiple assays increased steadily. Several devices are available affected, however, by limitations in terms of costs, size, complexity and portability. A compact low-cost SPRi biosensor based on a novel method for multi-analyte detection is presented. The prototype consists of a nanohole array biochip integrated with a compact optics and an elaboration system. A CMOS image sensor captures reflected light from the biochip surface irradiated by a 830 nm LED. The entire system is managed by an ARM9 processor. The biosensor was able to detect a ~10-5 RIU change in the refractive index without analyte receptors at a glycerol concentration equal to 0.2%. Results are available in 14 seconds on LCD display and immediately stored to external SD memory. Preliminary experiments confirmed the strong biosensor's usability in a wide range of applications and fieldsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
