Sample photofading or other causes of slow drift of the signal intensity can induce significant distortions in fluorescence spectra measured with wavelength scanning apparatus. These distortions are analyzed and quantitatively assessed in terms of photofading rate and scanning time. For a given measurement time, it is shown to be advantageous to average spectra over fast repeated scans instead of a single slow scan. Furthermore, an up-down scaling mode is shown to be preferable to an up-up mode. Experimental data, obtained on typical cytochemical samples, confirm the results of the analysis.
Minimization of photofading and drift-induced errors by spectrum scanning strategy
BUCETA SANDE DE FREITAS, MARIA ISABEL
1986-01-01
Abstract
Sample photofading or other causes of slow drift of the signal intensity can induce significant distortions in fluorescence spectra measured with wavelength scanning apparatus. These distortions are analyzed and quantitatively assessed in terms of photofading rate and scanning time. For a given measurement time, it is shown to be advantageous to average spectra over fast repeated scans instead of a single slow scan. Furthermore, an up-down scaling mode is shown to be preferable to an up-up mode. Experimental data, obtained on typical cytochemical samples, confirm the results of the analysis.File in questo prodotto:
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