In this work the fission-track dating method was applied for the identification of the provenance of 26 obsidian artefacts excavated from the Late Chalcolithic levels of the Tell Afis archaeological site (north-western Syria). Results of this study confirm the belonging of the Tell Afis site to the north-western Levantine province, since most artefacts originated from central Anatolian sources. Presence of some artefacts originated from the eastern Anatolian source area of Bingôl documents contacts also with the middle Euphrates river valley. A unique artefact showed unknown fission-track parameters and should be related to an unknown source. This result confirms the need of further field work aimed to enhance knowledge of the potential natural sources of raw material of this region.