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EnglishWe transformed synchronized mouse L-thymidine kinase deficient cells with a cloned thymidine kinase gene and carrier DNA at different times in the cell cycle. The frequency of resistant colonies was determined after growth in hypoxanthine-aminopterin-thymidine medium for two weeks (late expression) and the frequency of thymidine kinase positive cells was determined 72 hours after transformation (early expression) by incorporation of 3H-thymidine and autoradiography. The frequency of late expression was several-fold higher when cells were exposed to DNA during S-phase, whereas the frequency of early expression did not depend on the phase of the cell cycle.
DNA-mediated gene transfer is more efficient during S-phase of the cell cycle. / E. GIULOTTO; ISRAEL N.. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 118:1(1984), pp. 310-316.
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| Titolo: | DNA-mediated gene transfer is more efficient during S-phase of the cell cycle. | |
| Autori: | ||
| Data di pubblicazione: | 1984 | |
| Rivista: | ||
| Citazione: | DNA-mediated gene transfer is more efficient during S-phase of the cell cycle. / E. GIULOTTO; ISRAEL N.. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 118:1(1984), pp. 310-316. | |
| Abstract: | We transformed synchronized mouse L-thymidine kinase deficient cells with a cloned thymidine kinase gene and carrier DNA at different times in the cell cycle. The frequency of resistant colonies was determined after growth in hypoxanthine-aminopterin-thymidine medium for two weeks (late expression) and the frequency of thymidine kinase positive cells was determined 72 hours after transformation (early expression) by incorporation of 3H-thymidine and autoradiography. The frequency of late expression was several-fold higher when cells were exposed to DNA during S-phase, whereas the frequency of early expression did not depend on the phase of the cell cycle. | |
| Handle: | http://hdl.handle.net/11571/107048 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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