A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
Lactobacillus protoplast transformation. Plasmid 17, 73-75. / MORELLI L; COCCONCELLI PS; BOTTAZZI V; DAMIANI G; FERRETTI L; SGARAMELLA V. - In: PLASMID. - ISSN 0147-619X. - STAMPA. - 17(1987), pp. 73-75.
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Titolo: | Lactobacillus protoplast transformation. Plasmid 17, 73-75. |
Autori: | |
Data di pubblicazione: | 1987 |
Rivista: | |
Citazione: | Lactobacillus protoplast transformation. Plasmid 17, 73-75. / MORELLI L; COCCONCELLI PS; BOTTAZZI V; DAMIANI G; FERRETTI L; SGARAMELLA V. - In: PLASMID. - ISSN 0147-619X. - STAMPA. - 17(1987), pp. 73-75. |
Abstract: | A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%. |
Handle: | http://hdl.handle.net/11571/108140 |
Appare nelle tipologie: | 1.1 Articolo in rivista |