Lactobacillus protoplast transformation. Plasmid 17, 73-75.

A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.

Lactobacillus protoplast transformation. Plasmid 17, 73-75. / MORELLI L; COCCONCELLI PS; BOTTAZZI V; DAMIANI G; FERRETTI L; SGARAMELLA V. - In: PLASMID. - ISSN 0147-619X. - STAMPA. - 17(1987), pp. 73-75.



Titolo: Lactobacillus protoplast transformation. Plasmid 17, 73-75.
Autori: 
FERRETTI, LUCA
mostra contributor esterni 
Data di pubblicazione: 1987
Rivista: 
PLASMID  
Citazione: Lactobacillus protoplast transformation. Plasmid 17, 73-75. / MORELLI L; COCCONCELLI PS; BOTTAZZI V; DAMIANI G; FERRETTI L; SGARAMELLA V. - In: PLASMID. - ISSN 0147-619X. - STAMPA. - 17(1987), pp. 73-75.
Abstract: A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
Handle: http://hdl.handle.net/11571/108140
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