The draft genome sequence of Italian specimens of the Asian tiger mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae) was determined using a standard NGS (next generation sequencing) approach. The size of the assembled genome is comparable to that of Aedes aegypti; the two mosquitoes are also similar as far as the high content of repetitive DNA is concerned, most of which is made up of transposable elements. Although, based on BUSCO (Benchmarking Universal Single-Copy Orthologues) analysis, the genome assembly reported here contains more than 99% of protein-coding genes, several of those are expected to be represented in the assembly in a fragmented state. We also present here the annotation of several families of genes (tRNA genes, miRNA genes, the sialome, genes involved in chromatin condensation, sex determination genes, odorant binding proteins and odorant receptors). These analyses confirm that the assembly can be used for the study of the biology of this invasive vector of disease.

A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus

GASPERI, GIULIANO;GOMULSKI, LUDVIK;SAVINI, GRAZIA;MANNI, MOSE';SCOLARI, FRANCESCA;MALACRIDA, ANNA RODOLFA;ARCA', BRUNO;LOMBARDO, FABRIZIO;SACCONE, GIUSEPPE;Calvitti, Maurizio;FAVIA, GUIDO;LOUIS, CHRISTOS
2015-01-01

Abstract

The draft genome sequence of Italian specimens of the Asian tiger mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae) was determined using a standard NGS (next generation sequencing) approach. The size of the assembled genome is comparable to that of Aedes aegypti; the two mosquitoes are also similar as far as the high content of repetitive DNA is concerned, most of which is made up of transposable elements. Although, based on BUSCO (Benchmarking Universal Single-Copy Orthologues) analysis, the genome assembly reported here contains more than 99% of protein-coding genes, several of those are expected to be represented in the assembly in a fragmented state. We also present here the annotation of several families of genes (tRNA genes, miRNA genes, the sialome, genes involved in chromatin condensation, sex determination genes, odorant binding proteins and odorant receptors). These analyses confirm that the assembly can be used for the study of the biology of this invasive vector of disease.
2015
Molecular Biology & Genetics considers all aspects of basic and applied genetics, including molecular genetics, prokaryotic and eukaryotic gene expression, mechanisms of mutagenesis, structure, function and regulation of genetic material. Also included are resources concerned with clinical genetics, patterns of inheritance, genetic cause, and screening and treatment of disease. Resources dealing specifically with developmentally regulated gene expression, or with signal transduction pathways that modulate gene expression at the cellular level are excluded and are covered in the Cell and Developmental Biology category.
Esperti anonimi
Inglese
Internazionale
ELETTRONICO
109
5
207
220
14
BUSCO; Chikungunya; Dengue fever; Disease vector; Invasive species; NGS; Repetitive DNA; Transposable elements; WGS; Parasitology; Infectious Diseases; Public Health, Environmental and Occupational Health; Microbiology
http://www.maneyonline.com/doi/pdfplus/10.1179/2047773215Y.0000000031
31
info:eu-repo/semantics/article
262
Dritsou, Vicky; Topalis, Pantelis; Windbichler, Nikolai; Simoni, Alekos; Hall, Ann; Lawson, Daniel; Hinsley, Malcolm; Hughes, Daniel; Napolioni, Valer...espandi
1 Contributo su Rivista::1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/1108393
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