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Here, we report the three-dimensional structure and biochemical investigation of wild-type Kokobera virus helicase and of a mutant targeting a residue of motif Ia. This study has been carried out within the framework of the Vizier project (www.vizier-europe.org) that is aimed at the molecular characterization of the replicative machinery of RNA viruses. The comparative analysis of the flavivirus helicase structures is the first step towards the development of antiviral compounds interfering with the activity of these essential replicative enzymes.

Structure and biochemical analysis of Kokobera virus helicase.

SPERONI, SILVIA;DE COLIBUS, LUIGI;MATTEVI, ANDREA
2008-01-01

Abstract

Here, we report the three-dimensional structure and biochemical investigation of wild-type Kokobera virus helicase and of a mutant targeting a residue of motif Ia. This study has been carried out within the framework of the Vizier project (www.vizier-europe.org) that is aimed at the molecular characterization of the replicative machinery of RNA viruses. The comparative analysis of the flavivirus helicase structures is the first step towards the development of antiviral compounds interfering with the activity of these essential replicative enzymes.
2008
Biochemistry & Biophysics focuses on the structure and chemistry of biomolecules and covers all aspects of basic biochemistry/biophysics, including molecular structure, enzyme kinetics and protein-protein interaction; this category also contains cross-disciplinary resources focused on a specific class of biological molecules, e.g., nucleic acids, steroids, magnesium, growth factors, free radicals, bio-membranes, and peptides. Excluded are resources dealing with the application of biochemical techniques to specific topics listed elsewhere in CC/LS. Resources with a strong emphasis on the integration of biochemical pathways (such as signal transduction or molecular motors) at the cellular level are placed in the Cell & Developmental Biology category.
PROTEINS
sì
WOS:000252836300047
2-s2.0-38549123983
Sì, ma tipo non specificato
Inglese
Internazionale
STAMPA
70
1120
1123
4
18004778
flavivirus; structural biology; helicase
9
info:eu-repo/semantics/article
262
Speroni, Silvia; DE COLIBUS, Luigi; Mastrangelo, E; Gould, E; Coutard, B; Forrester, Nl; Blanc, S; Canard, B; Mattevi, Andrea
1 Contributo su Rivista::1.1 Articolo in rivista
none
1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/113346
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