Immune-mediated and neurodegenerative mechanisms are involved in multiple sclerosis (MS). Growing evidences highlight the role of HSP70 genes in the susceptibility of some neurological diseases. In this explorative study we analyzed a polymorphism (i.e. HSP70-hom rs2227956) of the gene HSPA1L, which encodes for the protein hsp70-hom. We sequenced the polymorphism by polymerase chain reaction (PCR), in 191MS patients and 365 healthy controls. The hsp70-hom protein expression was quantified by western blotting. Wereported a strong association between rs2227956 polymorphismandMS risk,which is independent fromthe association with HSP70-2 rs1061581, and a significant link between hsp70-hom protein expression and MS severity.
Heat shock protein 70-hom gene polymorphism and protein expression in multiple sclerosis
BOIOCCHI, CHIARA;MONTI, MARIA CRISTINA;OSERA, CECILIA;MALLUCCI, GIULIA;PISTONO, CRISTIANA;FERRARO, OTTAVIA ELEONORA;NOSARI, GUIDO;ROMANI, ALFREDO;CUCCIA, MARIACLARA;GOVONI, STEFANO;PASCALE, ALESSIA ANGELA;MONTOMOLI, CRISTINA;BERGAMASCHI, ROBERTO
2016-01-01
Abstract
Immune-mediated and neurodegenerative mechanisms are involved in multiple sclerosis (MS). Growing evidences highlight the role of HSP70 genes in the susceptibility of some neurological diseases. In this explorative study we analyzed a polymorphism (i.e. HSP70-hom rs2227956) of the gene HSPA1L, which encodes for the protein hsp70-hom. We sequenced the polymorphism by polymerase chain reaction (PCR), in 191MS patients and 365 healthy controls. The hsp70-hom protein expression was quantified by western blotting. Wereported a strong association between rs2227956 polymorphismandMS risk,which is independent fromthe association with HSP70-2 rs1061581, and a significant link between hsp70-hom protein expression and MS severity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
