This paper contains original data supporting the antibacterial activities of Gallium (Ga3þ)-doped pro-osteointegrative titanium alloys, obtained via Anodic Spark Deposition (ASD), as described in “The effect of silver or gallium doped titanium against the multi- drug resistant Acinetobacter baumannii” (Cochis et al. 2016) [1]. In this article we included an indirect cytocompatibility eva- luation towards Saos2 human osteoblasts and extended the microbial evaluation of the Ga3þ enriched titanium surfaces against the biofilm former Escherichia coli and Staphylococcus epi- dermidis strains. Cell viability was assayed by the Alamar Blue test, while bacterial viability was evaluated by the metabolic colori- metric 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bro- mide (MTT) assay. Finally biofilm morphology was analyzed by Scanning Electron Microscopy (SEM). Data regarding Ga3 þ activity were compared to Silver.
Data in support of Gallium (Ga3+) antibacterial activities to counteract E. coli and S. epidermidis biofilm formation onto pro-osteointegrative titanium surfaces
BLOISE, NORA;VISAI, LIVIA;BRUNI, GIOVANNA;
2016-01-01
Abstract
This paper contains original data supporting the antibacterial activities of Gallium (Ga3þ)-doped pro-osteointegrative titanium alloys, obtained via Anodic Spark Deposition (ASD), as described in “The effect of silver or gallium doped titanium against the multi- drug resistant Acinetobacter baumannii” (Cochis et al. 2016) [1]. In this article we included an indirect cytocompatibility eva- luation towards Saos2 human osteoblasts and extended the microbial evaluation of the Ga3þ enriched titanium surfaces against the biofilm former Escherichia coli and Staphylococcus epi- dermidis strains. Cell viability was assayed by the Alamar Blue test, while bacterial viability was evaluated by the metabolic colori- metric 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bro- mide (MTT) assay. Finally biofilm morphology was analyzed by Scanning Electron Microscopy (SEM). Data regarding Ga3 þ activity were compared to Silver.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.