Displacement of the previously bound, and quenched, fluorescent indicator (I) by coordination of the pyrophosphate ion to the two CuII centers of the receptor R results in the generation of the natural fluorescence of the indicator, and thus signals pyrophosphate recognition (see scheme). Discrimination from interfering anions (HPO42−, SO42−, NO3−, Cl−, NCO−, N3−, acetate, benzoate) can be achieved by tuning the indicator affinity towards the receptor.
Pyrophosphate Detection in Water by Fluorescence Competition Assays: Inducing Selectivity through the Choice of the Indicator.
FABBRIZZI, LUIGI;TAGLIETTI, ANGELO MARIA
2002-01-01
Abstract
Displacement of the previously bound, and quenched, fluorescent indicator (I) by coordination of the pyrophosphate ion to the two CuII centers of the receptor R results in the generation of the natural fluorescence of the indicator, and thus signals pyrophosphate recognition (see scheme). Discrimination from interfering anions (HPO42−, SO42−, NO3−, Cl−, NCO−, N3−, acetate, benzoate) can be achieved by tuning the indicator affinity towards the receptor.File in questo prodotto:
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