Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.

Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

BERTONE, VITTORIO;TARANTOLA, ELEONORA;BUCETA SANDE DE FREITAS, MARIA ISABEL
2017-01-01

Abstract

Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.
2017
Histochemistry of single molecules - Methods and protocols
Carlo Pellicciari, Marco Biggiogera
Experimental Biology covers a wide array of topics concerned with research in general biology and biological systems, including evolution, ecology, radiation biology, anatomy, general biology, and resources containing diverse topics in basic biology research. Resources on general biomedicine are excluded and are covered in the Medical Research: General Topics category. Resources with strong reliance on fields that fall outside of the core topics of Life sciences, such as biomedical engineering are placed in the Multidisciplinary category.
Comitato scientifico
Inglese
Internazionale
STAMPA
45
53
9
978-1-4939-6787-2
Humana Press - Springer Nature
New York
STATI UNITI D'AMERICA
Histochemistry, DPP-IV, Dipeptidyl-peptidase IV, rat liver
http://www.springer.com/us/book/9781493967872
2 Contributo in Volume::2.1 Contributo in volume (Capitolo o Saggio)
3
268
none
Bertone, Vittorio; Tarantola, Eleonora; BUCETA SANDE DE FREITAS, MARIA ISABEL
info:eu-repo/semantics/bookPart
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/1177719
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