The role of tyrosyl-DNA phosphodiesterase 2 (Tdp2) involved in the repair of 50-endblocking DNA lesions is still poorly explored in plants. To gain novel insights, Medicago truncatula suspension cultures overexpressing the MtTdp2a gene (Tdp2a-13C and Tdp2a-28 lines, respectively) and a control (CTRL) line carrying the empty vector were investigated. Transmission electron microscopy (TEM) revealed enlarged nucleoli (up to 44% expansion of the area, compared to CTRL), the presence of nucleolar vacuoles, increased frequency of multinucleolate cells (up to 4.3-fold compared to CTRL) and reduced number of ring-shaped nucleoli in Tdp2a-13C and Tdp2a-28 lines. Ultrastructural data suggesting for enhanced nucleolar activity in MtTdp2a- overexpressing lines were integrated with results from bromouridine incorporation. The latter revealed an increase of labeled transcripts in both Tdp2a-13C and Tdp2a-28 cells, within the nucleolus and in the extra-nucleolar region. MtTdp2a-overexpressing cells showed tolerance to etoposide, a selective inhibitor of DNA topoisomerase II, as evidenced by DNA diffusion assay. TEM analysis revealed etoposide-induced rearrangements within the nucleolus, resembling the nucleolar caps observed in animal cells under transcription impairment. Based on these findings it is evident that MtTdp2a-overexpression enhances nucleolar activity in plant cells.
Ultrastructural and molecular analyses reveal enhanced nucleolar activity in Medicago truncatula cells overexpressing the MtTdp2alpha gene
Macovei A;Faè M;Biggiogera MMembro del Collaboration Group
;Balestrazzi A
2018-01-01
Abstract
The role of tyrosyl-DNA phosphodiesterase 2 (Tdp2) involved in the repair of 50-endblocking DNA lesions is still poorly explored in plants. To gain novel insights, Medicago truncatula suspension cultures overexpressing the MtTdp2a gene (Tdp2a-13C and Tdp2a-28 lines, respectively) and a control (CTRL) line carrying the empty vector were investigated. Transmission electron microscopy (TEM) revealed enlarged nucleoli (up to 44% expansion of the area, compared to CTRL), the presence of nucleolar vacuoles, increased frequency of multinucleolate cells (up to 4.3-fold compared to CTRL) and reduced number of ring-shaped nucleoli in Tdp2a-13C and Tdp2a-28 lines. Ultrastructural data suggesting for enhanced nucleolar activity in MtTdp2a- overexpressing lines were integrated with results from bromouridine incorporation. The latter revealed an increase of labeled transcripts in both Tdp2a-13C and Tdp2a-28 cells, within the nucleolus and in the extra-nucleolar region. MtTdp2a-overexpressing cells showed tolerance to etoposide, a selective inhibitor of DNA topoisomerase II, as evidenced by DNA diffusion assay. TEM analysis revealed etoposide-induced rearrangements within the nucleolus, resembling the nucleolar caps observed in animal cells under transcription impairment. Based on these findings it is evident that MtTdp2a-overexpression enhances nucleolar activity in plant cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
