A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes.
A Catalytic and Selective Scissoring Molecular Tool for Quadruplex Nucleic Acids
Doria, FilippoWriting – Original Draft Preparation
;Pirota, ValentinaInvestigation
;Grande, VincenzoSoftware
;Bergamaschi, GretaFormal Analysis
;Amendola, ValeriaFormal Analysis
;Freccero, Mauro
Writing – Review & Editing
2018-01-01
Abstract
A copper complex embedded in the structure of a water-soluble naphthalene diimide has been designed to bind and cleave G-quadruplex DNA. We describe the properties of this ligand, including its catalytic activity in the generation of ROS. FRET melting, CD, NMR, gel sequencing, and mass spectrometry experiments highlight a unique and unexpected selectivity in cleaving G-quadruplex sequences. This selectivity relies both on the binding affinity and structural features of the targeted G-quadruplexes.File in questo prodotto:
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