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Steady-state and pulse-chase collagen analyses are powerful approaches to investigate in vitro the structure of collagen and its kinetic of secretion, respectively. The electrophoretic analysis of purified 3H-proline-labeled collagen allows to determine the nature and post translational modifications of its α chains, whereas short-pulse labeling can be used to follow collagen secretion over time.

Steady-state and pulse-chase analyses of fibrillar collagen

Forlino A.;Tonelli F.;Besio R.
2019-01-01

Abstract

Steady-state and pulse-chase collagen analyses are powerful approaches to investigate in vitro the structure of collagen and its kinetic of secretion, respectively. The electrophoretic analysis of purified 3H-proline-labeled collagen allows to determine the nature and post translational modifications of its α chains, whereas short-pulse labeling can be used to follow collagen secretion over time.
2019
Methods in Molecular Biology
2-s2.0-85062424599
Esperti anonimi
Inglese
Internazionale
STAMPA
1952
45
53
9
978-1-4939-9132-7
978-1-4939-9133-4
Humana Press Inc.
Collagen; Osteogenesis imperfecta; Post translational modifications; Pulse-chase; Steady-state; Animals; Electrophoresis, Polyacrylamide Gel; Fibrillar Collagens; Fibroblasts; Humans; Proline; Protein Processing, Post-Translational; Tritium; Urea
http://www.springer.com/series/7651
no
2 Contributo in Volume::2.1 Contributo in volume (Capitolo o Saggio)
3
268
none
Forlino, A.; Tonelli, F.; Besio, R.
info:eu-repo/semantics/bookPart
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/1278787
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