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A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucleotidase and transferase activities of cytosolic 5'-nucleotidase III is presented. This electrophoretic approach was successfully applied to human erythrocyte lysates to monitor the enzyme activities indicated above, using either physiological substrate or the nucleoside analogue 3'-azido-3'-deoxythymidine as the phosphate acceptor.

Pyrimidine 5'-nucleotidase activities detected in real samples by means of micellar electrokinetic chromatography.

CHIARELLI, LAURENT;VALENTINI, GIOVANNA;VIGLIO, SIMONA;IADAROLA, PAOLO
2004-01-01

Abstract

A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucleotidase and transferase activities of cytosolic 5'-nucleotidase III is presented. This electrophoretic approach was successfully applied to human erythrocyte lysates to monitor the enzyme activities indicated above, using either physiological substrate or the nucleoside analogue 3'-azido-3'-deoxythymidine as the phosphate acceptor.
2004
Biochemistry & Biophysics focuses on the structure and chemistry of biomolecules and covers all aspects of basic biochemistry/biophysics, including molecular structure, enzyme kinetics and protein-protein interaction; this category also contains cross-disciplinary resources focused on a specific class of biological molecules, e.g., nucleic acids, steroids, magnesium, growth factors, free radicals, bio-membranes, and peptides. Excluded are resources dealing with the application of biochemical techniques to specific topics listed elsewhere in CC/LS. Resources with a strong emphasis on the integration of biochemical pathways (such as signal transduction or molecular motors) at the cellular level are placed in the Cell & Developmental Biology category.
ELECTROPHORESIS
sì
000224649500029
2-s2.0-8344278023
Esperti anonimi
Inglese
Internazionale
STAMPA
25
18
3270
3276
7
15472954
Cytosolic 5'-nucleotidase III; Enzymatic activity; Human erythrocytes
http://www3.interscience.wiley.com/journal/109674760/abstract
no
7
info:eu-repo/semantics/article
262
Zanone, C.; Chiarelli, Laurent; Valentini, Giovanna; Perani, E.; Annovazzi, L.; Viglio, Simona; Iadarola, Paolo
1 Contributo su Rivista::1.1 Articolo in rivista
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1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/132153
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