Screening for the plasmid content of 94 strains belonging to ten species of thermophilic bacilli mainly referred toBacillus stearothermophilus was carried out. Twenty-seven of the strains tested were found to harbor one or more plasmids ranging in size from 1.7 to>50 kb. The physical map of the smallest plasmid, pBC1, is reported. Southern hybridization experiments showed that pBC1 hybridized strongly with the other small plasmids, weakly with medium-sized plasmids, and not at all with the largest plasmids and chromosomal DNA. pBC1 was cloned into pHV14 and pJH101 vectors, and the chimeric plasmids obtained were used to transformEscherichia coli andBacillus subtilis.
Plasmid screening in thermophilic Bacillus: Physical characterization and molecular cloning
DE ROSSI, EDDA;RICCARDI, GIOVANNA;
1989-01-01
Abstract
Screening for the plasmid content of 94 strains belonging to ten species of thermophilic bacilli mainly referred toBacillus stearothermophilus was carried out. Twenty-seven of the strains tested were found to harbor one or more plasmids ranging in size from 1.7 to>50 kb. The physical map of the smallest plasmid, pBC1, is reported. Southern hybridization experiments showed that pBC1 hybridized strongly with the other small plasmids, weakly with medium-sized plasmids, and not at all with the largest plasmids and chromosomal DNA. pBC1 was cloned into pHV14 and pJH101 vectors, and the chimeric plasmids obtained were used to transformEscherichia coli andBacillus subtilis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
