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EnglishA rapid and reliable protocol was developed to produce chemical degradation of the RNA in vitro. Cell line MDA-MB-453 was used as source of RNA and hydrolysis in water at 70 °C was performed. The outcome was monitored by RT-qPCR of two housekeeping targets and a significant correlation between the Ct and the extent of molecular damage of the template was found. A molecular degradation index of the RNA is proposed.
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| Titolo: | Reliability of RT-qPCR from degraded RNA samples: An in vitro model |
| Autori: | |
| Data di pubblicazione: | 2015 |
| Rivista: | |
| Abstract: | A rapid and reliable protocol was developed to produce chemical degradation of the RNA in vitro. Cell line MDA-MB-453 was used as source of RNA and hydrolysis in water at 70 °C was performed. The outcome was monitored by RT-qPCR of two housekeeping targets and a significant correlation between the Ct and the extent of molecular damage of the template was found. A molecular degradation index of the RNA is proposed. |
| Handle: | http://hdl.handle.net/11571/1347755 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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