Acid soluble type I collagen was prepared from foetal and adult bovine tendon and skin and from adult bovine cornea. The degree of hydroxylysine glycosylation and the hydroxylysine di-to monoglycoside ratio as well as the "in vivo" fibril diameters, were shown to be tissue and age-dependent. Fibrils of type I collagens were reconstituted "in vitro" monitoring at 313 nm. The fibrils obtained were examined by electron microscopy. It was shown that the "in vitro" lateral growth of collagen fibrils leads to the formation of fibrils with maximum diameters which may be correlated to those of the corresponding native fibrils. Moreover it is suggested that one of the factors controlling the lateral growth of collagen may be at the level of hydroxylysine glycosylation.

"In vitro" fibril formation of type I collagen from different sources: biochemical and morphological aspects. / Valli M; Leonardi L; Strocchi R; Tenni R; Guizzardi S; Ruggeri A; Balduini C.. - In: CONNECTIVE TISSUE RESEARCH. - ISSN 0300-8207. - STAMPA. - 15:4(1986), pp. 235-244.

"In vitro" fibril formation of type I collagen from different sources: biochemical and morphological aspects.

VALLI, MAURIZIA;TENNI, RUGGERO;BALDUINI, CESARE
1986

Abstract

Acid soluble type I collagen was prepared from foetal and adult bovine tendon and skin and from adult bovine cornea. The degree of hydroxylysine glycosylation and the hydroxylysine di-to monoglycoside ratio as well as the "in vivo" fibril diameters, were shown to be tissue and age-dependent. Fibrils of type I collagens were reconstituted "in vitro" monitoring at 313 nm. The fibrils obtained were examined by electron microscopy. It was shown that the "in vitro" lateral growth of collagen fibrils leads to the formation of fibrils with maximum diameters which may be correlated to those of the corresponding native fibrils. Moreover it is suggested that one of the factors controlling the lateral growth of collagen may be at the level of hydroxylysine glycosylation.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11571/141839
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