RGB-Detector: A Smart, Low-Cost Device for Reading RGB Indexes of Microfluidic Paper-Based Analytical Devices

A user-friendly, low-cost detector able to read the RGB indexes of microfluidic paper-based analytical devices (mu PADs) was developed. The RGB-detector was built with 3D printing using PLA+ and reused Li-ion batteries. It is Arduino-based, which provides an easy interface between the sensor TCS3200, which reads the quadratic wave of the times corresponding to the RGB numbers, the Arduino itself, whose software translates the times into RGB values, and the touchscreen display, NX3224T028, which shows the results. This detector permits multi-sample analysis since it has a sample holder that can keep up to six mu PADs simultaneously and rotate after the display's request. This work shows how the readings of the RGB indexes by the proposed RGB-detector implement the measurements' reproducibility. As a proof-of-concept, the RGB-detector application to a green array of mu PADs for pH measurement coupled with chemometric analysis allowed us to achieve good results in terms of precision and agreement with the pH values measured by a classical pH-meter.