The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

Vangelista L.
2006-01-01

Abstract

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-Fcεe RI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/1472722
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