Abstract BM examination is essential in the staging of NHL pts. Few studies have compared BM histologic findings with results of flow cytometric (FC) analysis. We analysed the incidence and patterns of histologic BM involvement in a series of 753 pts with NHL. For 498 pts a concurrent FC analysis on BM was available. Histologic involvement was detected at diagnosis in 311/753 (41%) pts. By FC, BM involvement was clearly detected in 150/498 (30%). After excluding 12 cases with equivocal histology, concordance between the 2 methods was detected in 411 (85%) cases (27% BMB+/FC+; 58% BMB-/FC-) while discordance was present in 75 (15%) (p<0.001): 58 cases (12%) were BMB+/FC- and 17 (3%) were BMB-/FC+. Discordance was more frequent in FL and in LPL. These data demonstrate that the two methods are comparable in qualitative assessment of BM involvement in NHL, with the exception of FL and LPL. In FL, DLBCL and LPL, FC underestimates the extent of infiltrate with respect to histology.

Assessment of bone marrow involvement in non-Hodgkin's lymphomas: comparison between histology and flow cytometry.

MERLI, MICHELE;ARCAINI, LUCA;RATTOTTI, SARA;PASSAMONTI, FRANCESCO;LUCIONI, MARCO;RIZZI, SILVIA;PAULLI, MARCO;LAZZARINO, MARIO
2010

Abstract

Abstract BM examination is essential in the staging of NHL pts. Few studies have compared BM histologic findings with results of flow cytometric (FC) analysis. We analysed the incidence and patterns of histologic BM involvement in a series of 753 pts with NHL. For 498 pts a concurrent FC analysis on BM was available. Histologic involvement was detected at diagnosis in 311/753 (41%) pts. By FC, BM involvement was clearly detected in 150/498 (30%). After excluding 12 cases with equivocal histology, concordance between the 2 methods was detected in 411 (85%) cases (27% BMB+/FC+; 58% BMB-/FC-) while discordance was present in 75 (15%) (p<0.001): 58 cases (12%) were BMB+/FC- and 17 (3%) were BMB-/FC+. Discordance was more frequent in FL and in LPL. These data demonstrate that the two methods are comparable in qualitative assessment of BM involvement in NHL, with the exception of FL and LPL. In FL, DLBCL and LPL, FC underestimates the extent of infiltrate with respect to histology.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/213664
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