Aim of the study: To investigate the antimicrobial properties of Phyllanthus muellerianus (Kuntze) Excell (family Euphorbiaceae) stem bark used in Cameroon by pygmies Baka as a remedy for wound healing and tetanus. Materials and methods: Aqueous and methanol extracts with and without defatting treatment, were prepared and their activity against Clostridium sporogenes ATCC 3584, Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, Streptococcus pyogenes ATCC 19615, Escherichia coli ATCC 10536, Candida albicans ATCC 10231, was evaluated on the basis of the minimum inhibitory concentration (MIC) and the minimum bactericidal-fungicidal concentration (MBC-MFC) by the macrodilution method. Results: Water extract showed a weak activity against Clostridium sporogenes ATCC 3584 (MIC 900 µg/mL) but resulted almost inactive against all the other microorganisms; the defatted methanol extract, almost inactive against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, Candida albicans ATCC 10231 exhibited a very interesting activity against the other tested micro-organisms, particularly against Clostridium sporogenes ATCC 3584 (MIC 100 µg/mL). Conclusions: The activity against streptococci and Clostridium sporogenes ATCC 3584, showed by stem bark extracts of Phyllanthus muellerianus, traditionally used by pygmies Baka to treat wound infections and tetanus, is reported for the first time.
Antimicrobial properties of stem bark extracts from Phyllanthus muellerianus (Kuntze) Excell
BRUSOTTI, GLORIA;GRISOLI, PIETRO;DACARRO, CESARE;CACCIALANZA, GABRIELE
2011-01-01
Abstract
Aim of the study: To investigate the antimicrobial properties of Phyllanthus muellerianus (Kuntze) Excell (family Euphorbiaceae) stem bark used in Cameroon by pygmies Baka as a remedy for wound healing and tetanus. Materials and methods: Aqueous and methanol extracts with and without defatting treatment, were prepared and their activity against Clostridium sporogenes ATCC 3584, Staphylococcus aureus ATCC 6538, Streptococcus mutans ATCC 25175, Streptococcus pyogenes ATCC 19615, Escherichia coli ATCC 10536, Candida albicans ATCC 10231, was evaluated on the basis of the minimum inhibitory concentration (MIC) and the minimum bactericidal-fungicidal concentration (MBC-MFC) by the macrodilution method. Results: Water extract showed a weak activity against Clostridium sporogenes ATCC 3584 (MIC 900 µg/mL) but resulted almost inactive against all the other microorganisms; the defatted methanol extract, almost inactive against Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, Candida albicans ATCC 10231 exhibited a very interesting activity against the other tested micro-organisms, particularly against Clostridium sporogenes ATCC 3584 (MIC 100 µg/mL). Conclusions: The activity against streptococci and Clostridium sporogenes ATCC 3584, showed by stem bark extracts of Phyllanthus muellerianus, traditionally used by pygmies Baka to treat wound infections and tetanus, is reported for the first time.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
