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Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques

BOTTONE, MARIA GRAZIA;SANTIN, GIADA;SOLDANI, CRISTIANA;VENERONI, PAOLA;SCOVASSI, ANNA;
2012-01-01
2012
Cell & Developmental Biology contains resources in biochemistry, molecular biology, biophysics, physiology, and pharmacology that have a specific emphasis on cellular function in eukaryotic systems. Topics of particular importance include receptor biology and signal transduction, regulation of gene expression at the cellular level, developmental genetics, developmental biology and morphogenesis, and cell-environment interactions. Resources concentrated on molecular biochemistry and molecular regulation of gene expression, as well as microscopic or histological analysis of cell or tissue samples are excluded.
EUROPEAN JOURNAL OF HISTOCHEMISTRY
sì
WOS:000303944500004
2-s2.0-84856033501
Sì, ma tipo non specificato
Inglese
Internazionale
ELETTRONICO
56
1
20
23
22472892
Poly(ADP-ribose) polymerases are a family of enzymes that catalyze the conversion of NAD+ into ADP-ribose. Among them, Tankyrases have been found to bind to centrosome, mitotic spindle and microsome proteins, in the cytoplasm, and to telomeres in the nucleus, where they play a relevant role in telomere metabolism. However, their precise intracellular localization during interphase has not been so far fully elucidated. We investigated this aspect in situ by double immunofluorescence experiments using antibodies recognizing Tankyrases 1-2 or other proteins residing in specific organelles (Golgi apparatus, mitochondria, lysosomes, endoplasmic reticulum). We used HeLa cells as a model system in vitro, before and after treatment with either actinomycin D or etoposide, to also investigate the possible relocation of Tankyrases during apoptosis. We observed that Tankyrases are distributed both in the nucleus and in the cytoplasm; in this latter compartment, they were found to colocate with the Golgi apparatus but never with the mitochondria; a pool of Tankyrases also colocates with the endoplasmic reticulum and lysosomes. Interestingly, in cells with clear signs of apoptosis, Tankyrases were detectable in the cytoplasmic blebs: this suggests that they are not massively cleaved during apoptosis and persist in the largely heterogeneous apoptotic remnants which are known to contain components of cytoplasmic and nuclear origin.
apoptosis; confocal microscopy; immunocytochemistry; organelles; PARP; Tankyrases.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352133/
6
info:eu-repo/semantics/article
262
Bottone, MARIA GRAZIA; Santin, Giada; Soldani, Cristiana; Veneroni, Paola; Scovassi, Anna; C., Alpini
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1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/375995
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