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Microrough, doubly acid etched titanium surfaces (Ti) were further modified by amination and covalent coupling of fibrillar collagen type I (ColTi). Human Mesenchymal Cells (HMC) adhesion and growth, and relevant osteogenic differentiation in nonosteogenic (basal) medium were evaluated by fluorescence microscopy, scanning electron microscopy, and RT-PCR for a three-week period. Results show strongly enhanced HMC adhesion and cell density at short experimental time on ColTi, together with complete spreading of the cell body over the microrough surface topography. RT-PCR analysis of several genes involved in osteogenesis indicate, since the first week of culturing, significant progression of HMC on ColTi along the osteogenic pathway. These results indicate that the adopted process of surface immobilization of collagen, mandatory to impart collagenase resistance in implant sites, does not impair biospecific interactions between HMC and collagen. Thus, it is possible to upgrade properties arising from the control of Ti surfaces topography by surface-chemistry driven enhanced recruitment of precursor osteogenic cells and pro-osteogenic stimula.

Gene expression of markers of osteogenic differentiation of human mesenchymal cells on collagen I-modified microrough titanium surfaces.

RODRIGUEZ Y BAENA, RUGGERO
2011-01-01

Abstract

Microrough, doubly acid etched titanium surfaces (Ti) were further modified by amination and covalent coupling of fibrillar collagen type I (ColTi). Human Mesenchymal Cells (HMC) adhesion and growth, and relevant osteogenic differentiation in nonosteogenic (basal) medium were evaluated by fluorescence microscopy, scanning electron microscopy, and RT-PCR for a three-week period. Results show strongly enhanced HMC adhesion and cell density at short experimental time on ColTi, together with complete spreading of the cell body over the microrough surface topography. RT-PCR analysis of several genes involved in osteogenesis indicate, since the first week of culturing, significant progression of HMC on ColTi along the osteogenic pathway. These results indicate that the adopted process of surface immobilization of collagen, mandatory to impart collagenase resistance in implant sites, does not impair biospecific interactions between HMC and collagen. Thus, it is possible to upgrade properties arising from the control of Ti surfaces topography by surface-chemistry driven enhanced recruitment of precursor osteogenic cells and pro-osteogenic stimula.
2011
The Dentistry/Oral Surgery & Medicine category covers resources concerned with all aspects of dental science and practice including dental implants and dental materials. Specialties such as orthodontics, periodontology, endodontics, prosthodontics, and pediatric dentistry are also included. Oral Surgery & Medicine resources are concerned with basic, applied, and clinical aspects of oral infections and diseases, including their epidemiology, diagnosis, treatment, and rehabilitation. Specialties such as oral pathology/biology, oral epidemiology, oral rehabilitation, and oral implants are also included. Facial pain and craniomandibular resources are also covered in this category.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A
sì
WOS:000285428700019
2-s2.0-78650433784
Esperti anonimi
Inglese
Internazionale
STAMPA
96
2
449
455
7
21171164
The Journal of Biomedical Materials Research is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys, polymers, ceramics, and reprocessed animal and human tissues in surgery, dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society For Biomaterials (USA) , the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials.
Animals, Biological Markers; metabolism, Cell Adhesion; drug effects, Cell Differentiation; drug effects/genetics, Cell Nucleus; drug effects/metabolism, Collagen Type I; pharmacology, Gene Expression Regulation; drug effects, Humans, Mesoderm; cytology/drug effects/metabolism/ultrastructure, Microscopy; Fluorescence, Osteogenesis; drug effects/genetics, Reverse Transcriptase Polymerase Chain Reaction, Surface Properties; drug effects, Sus scrofa, Titanium; pharmacology
http://dx.doi.org/10.1002/jbm.a.32948
5
info:eu-repo/semantics/article
262
Marco, Morra; Clara, Cassinelli; Giuseppe, Cascardo; Daniele, Bollati; RODRIGUEZ Y BAENA, Ruggero
1 Contributo su Rivista::1.1 Articolo in rivista
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1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/384508
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