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EnglishWe have investigated at a cytological and molecular level, the effects of gene transfer in an in vitro model system. We have used NIH 3T3 as recipient cells, a YAC carrying the neo gene as a vector and lipofection and electroporation as delivery systems. In the stable transformants obtained we have evaluated the following biological endpoints: a) distribution of chromosome numbers, b) chromosome aberrations, c) sister chromatid exchanges, d) DNA fingerprints, e) number and localisation of the integration sites, f) neo-gene expression.
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| Titolo: | Gene transfection in mammlian cells: an evaluation of the potential mutagenic effects |
| Autori: | |
| Data di pubblicazione: | 1997 |
| Rivista: | |
| Abstract: | We have investigated at a cytological and molecular level, the effects of gene transfer in an in vitro model system. We have used NIH 3T3 as recipient cells, a YAC carrying the neo gene as a vector and lipofection and electroporation as delivery systems. In the stable transformants obtained we have evaluated the following biological endpoints: a) distribution of chromosome numbers, b) chromosome aberrations, c) sister chromatid exchanges, d) DNA fingerprints, e) number and localisation of the integration sites, f) neo-gene expression. |
| Handle: | http://hdl.handle.net/11571/430963 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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