We have investigated at a cytological and molecular level, the effects of gene transfer in an in vitro model system. We have used NIH 3T3 as recipient cells, a YAC carrying the neo gene as a vector and lipofection and electroporation as delivery systems. In the stable transformants obtained we have evaluated the following biological endpoints: a) distribution of chromosome numbers, b) chromosome aberrations, c) sister chromatid exchanges, d) DNA fingerprints, e) number and localisation of the integration sites, f) neo-gene expression.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
Titolo: | Gene transfection in mammlian cells: an evaluation of the potential mutagenic effects | |
Autori: | ||
Data di pubblicazione: | 1997 | |
Rivista: | ||
Abstract: | We have investigated at a cytological and molecular level, the effects of gene transfer in an in vitro model system. We have used NIH 3T3 as recipient cells, a YAC carrying the neo gene as a vector and lipofection and electroporation as delivery systems. In the stable transformants obtained we have evaluated the following biological endpoints: a) distribution of chromosome numbers, b) chromosome aberrations, c) sister chromatid exchanges, d) DNA fingerprints, e) number and localisation of the integration sites, f) neo-gene expression. | |
Handle: | http://hdl.handle.net/11571/430963 | |
Appare nelle tipologie: | 1.1 Articolo in rivista |