IRIS

The possible use of polarized-immunofluorescence to evaluate aminoglycoside-modifying enzyme activities has been investigated. The TDX instrument was used to determine the residual quantity of aminoglycoside following inactivation during the assay procedure. Through the spectrum of the modified aminoglycoside antibiotics the enzymes encoded on eleven R-factors from clinical isolates of Enterobacteriaceae have been identified.

Fluorescence polarization immunoassay to determine aminoglycoside modifying enzymes activity.

PAGANI, LAURA;PERDUCA, MARGHERITA;ROMERO, EGIDIO
1986-01-01

Abstract

The possible use of polarized-immunofluorescence to evaluate aminoglycoside-modifying enzyme activities has been investigated. The TDX instrument was used to determine the residual quantity of aminoglycoside following inactivation during the assay procedure. Through the spectrum of the modified aminoglycoside antibiotics the enzymes encoded on eleven R-factors from clinical isolates of Enterobacteriaceae have been identified.
1986
Microbiology covers the biology and biochemistry of microorganisms, bacterial, viral, and parasitic, as well as the medical implications and treatments of the subset of these organisms known to cause disease in humans and/or animals. Biotechnology applications of microorganisms for basic science or clinical use are also covered. Resources that emphasize immune response to pathogens and its modulation by clinical intervention are excluded and are covered in the Immunology category.
MICROBIOLOGICA
sì
A1986E426300004
Sì, ma tipo non specificato
Italiano
Nazionale
STAMPA
9
423
430
3022111
Acetyltransferases; analysis, Aminoglycosides; metabolism, Anti-Bacterial Agents; metabolism, Enterobacteriaceae; enzymology/genetics, Escherichia coli; enzymology/genetics, Fluorescence Polarization, Fluorescent Antibody Technique, Nucleotidyltransferases; analysis, Phosphotransferases; analysis, R Factors, Substrate Specificity, Transferases; analysis
4
info:eu-repo/semantics/article
262
Pagani, Laura; Perduca, Margherita; V., Mills; Romero, Egidio
1 Contributo su Rivista::1.1 Articolo in rivista
none
1.1 Articolo in rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/430975
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 0
social impact