Here we describe the extraction from soil of the major photo-transformation products (PTPs) of Enrofloxacin (ENR) and Danofloxacin (DAN), two fluoroquinolones (FQs) widely employed in veterinary medicine and further of growing environmental concern, as their byproducts PTPs were shown to maintain high antibacterial activity. The microwave-assisted extraction (MAE) earlier developed for FQs determination and, based on an alkaline aqueous solution of Mg(II) as analytes complexing agent, was applied to agricultural soil samples fortified with different amounts of PTPs, along with residual parent compounds (53-1000 ng g-1 for ENR, 24-148 ng g-1 for DAN). The PTPs, obtained by irradiation of thin layers of the two drugs, after extraction were separated and quantified by HPLC-FD. Good recovery (70-130%) and precision (RSDs 1-6% in repeatability and 9-22% in reproducibility) were gained by the overall analytical procedure. The method was applied for the first time to study the in-soil lifecycle of ENR and DAN photoproductsPTPs , generated in the matrix by irradiation under natural sunlight, at environmentally -significant concentrations. Results indicated that soil-adsorbed FQ byproducts PTPs are themselves liable to photodegradation and show lifetimes comparable with those of parent compounds.
Microwave-assisted extraction and determination of Enrofloxacin and Danofloxacin photo-transformation products in soil
SPELTINI, ANDREA;STURINI, MICHELA;MARASCHI, FEDERICA;PROFUMO, ANTONELLA;ALBINI, ANGELO
2012-01-01
Abstract
Here we describe the extraction from soil of the major photo-transformation products (PTPs) of Enrofloxacin (ENR) and Danofloxacin (DAN), two fluoroquinolones (FQs) widely employed in veterinary medicine and further of growing environmental concern, as their byproducts PTPs were shown to maintain high antibacterial activity. The microwave-assisted extraction (MAE) earlier developed for FQs determination and, based on an alkaline aqueous solution of Mg(II) as analytes complexing agent, was applied to agricultural soil samples fortified with different amounts of PTPs, along with residual parent compounds (53-1000 ng g-1 for ENR, 24-148 ng g-1 for DAN). The PTPs, obtained by irradiation of thin layers of the two drugs, after extraction were separated and quantified by HPLC-FD. Good recovery (70-130%) and precision (RSDs 1-6% in repeatability and 9-22% in reproducibility) were gained by the overall analytical procedure. The method was applied for the first time to study the in-soil lifecycle of ENR and DAN photoproductsPTPs , generated in the matrix by irradiation under natural sunlight, at environmentally -significant concentrations. Results indicated that soil-adsorbed FQ byproducts PTPs are themselves liable to photodegradation and show lifetimes comparable with those of parent compounds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
