Non-dividing X-irradiated carrot uspension cells were used as feeder cells to support the division of Nicotiana protoplasts and cells plated at low densities. Pigmented carrot cells have been employed beacuse they are easily recognized when occasional divison and colony formation occurred in the feeder layer. Several parameters wich influenced the plating efficiency have been analyzed. Optimal conditions, which allowed a high final plating efficiency of up to 70%, in the case of Niocotiana tabacum cv Xanthi protoplasts, have been established.
Utilization of irradiated carrot cell suspensions as feeder layer for cultured Nicotiana cells and protoplasts.
CELLA, RINO;
1980-01-01
Abstract
Non-dividing X-irradiated carrot uspension cells were used as feeder cells to support the division of Nicotiana protoplasts and cells plated at low densities. Pigmented carrot cells have been employed beacuse they are easily recognized when occasional divison and colony formation occurred in the feeder layer. Several parameters wich influenced the plating efficiency have been analyzed. Optimal conditions, which allowed a high final plating efficiency of up to 70%, in the case of Niocotiana tabacum cv Xanthi protoplasts, have been established.File in questo prodotto:
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