Image analysis can contribute to those fields of cytogenetics that are influenced most by subjectivity, especially evaluation of chromosome regions that are histochemically polymorphic. C-banding of human or primate chromosomes may be used as a typical example of this concept. In addition, using it quantitatively it is useful in studies of population cytogenetics of man and Primates. Therefore, the aim of this study was to determine the best conditions for measurement by image analysis of C-positive regions in a sample of 29 normal subjects. We looked for relationships of chromosomal C-positive areas with the dimensions of the corresponding metaphases. Finally, we suggest some criteria for potential use of C-banding in population and comparative studies.
Image analysis as a simple and useful tool in evaluating the constitutive heterochromatin (C-banding) of human chromosomes
FORMENTI, DANIELE;
1993-01-01
Abstract
Image analysis can contribute to those fields of cytogenetics that are influenced most by subjectivity, especially evaluation of chromosome regions that are histochemically polymorphic. C-banding of human or primate chromosomes may be used as a typical example of this concept. In addition, using it quantitatively it is useful in studies of population cytogenetics of man and Primates. Therefore, the aim of this study was to determine the best conditions for measurement by image analysis of C-positive regions in a sample of 29 normal subjects. We looked for relationships of chromosomal C-positive areas with the dimensions of the corresponding metaphases. Finally, we suggest some criteria for potential use of C-banding in population and comparative studies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
