Diospyros bipindensis (Gürke) stem barks are used in Cameroon by pygmies Baka for the treatment of pulmonary diseases. One hypothesis was that this type of diseases could be caused by fungi. Thus the antifungal activity of the traditional preparation as well as that of different extracts was tested against Candida albicans. Some extracts presented a significant antifungal activity. The bioguided isolation was undertaken using HPLC-microfractionation in 96 well plates combined with bioautography in order to localize the active compounds in the HPLC profiling of the extracts. In a second step, medium pressure chromatography was used to isolate the active compounds. Using this approach seventeen compounds were isolated, nine of them are new natural products. The structures of the isolated compounds were elucidated by classical spectroscopic methods including UV, NMR and HR-MS.
ANTIFUNGAL COMPOUNDS ISOLATED FROM DIOSPYROS BIPINDENSIS
CESARI, ILARIA;BRUSOTTI, GLORIA;CACCIALANZA, GABRIELE;
2012-01-01
Abstract
Diospyros bipindensis (Gürke) stem barks are used in Cameroon by pygmies Baka for the treatment of pulmonary diseases. One hypothesis was that this type of diseases could be caused by fungi. Thus the antifungal activity of the traditional preparation as well as that of different extracts was tested against Candida albicans. Some extracts presented a significant antifungal activity. The bioguided isolation was undertaken using HPLC-microfractionation in 96 well plates combined with bioautography in order to localize the active compounds in the HPLC profiling of the extracts. In a second step, medium pressure chromatography was used to isolate the active compounds. Using this approach seventeen compounds were isolated, nine of them are new natural products. The structures of the isolated compounds were elucidated by classical spectroscopic methods including UV, NMR and HR-MS.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
