Diospyros bipindensis (Gürke) stem barks are used in Cameroon by pygmies Baka for the treatment of pulmonary diseases. One hypothesis was that this type of diseases could be caused by fungi. Thus the antifungal activity of the traditional preparation as well as that of different extracts was tested against Candida albicans. Some extracts presented a significant antifungal activity. The bioguided isolation was undertaken using HPLC-microfractionation in 96 well plates combined with bioautography in order to localize the active compounds in the HPLC profiling of the extracts. In a second step, medium pressure chromatography was used to isolate the active compounds. Using this approach seventeen compounds were isolated, nine of them are new natural products. The structures of the isolated compounds were elucidated by classical spectroscopic methods including UV, NMR and HR-MS.

ANTIFUNGAL COMPOUNDS ISOLATED FROM DIOSPYROS BIPINDENSIS

CESARI, ILARIA;BRUSOTTI, GLORIA;CACCIALANZA, GABRIELE;
2012-01-01

Abstract

Diospyros bipindensis (Gürke) stem barks are used in Cameroon by pygmies Baka for the treatment of pulmonary diseases. One hypothesis was that this type of diseases could be caused by fungi. Thus the antifungal activity of the traditional preparation as well as that of different extracts was tested against Candida albicans. Some extracts presented a significant antifungal activity. The bioguided isolation was undertaken using HPLC-microfractionation in 96 well plates combined with bioautography in order to localize the active compounds in the HPLC profiling of the extracts. In a second step, medium pressure chromatography was used to isolate the active compounds. Using this approach seventeen compounds were isolated, nine of them are new natural products. The structures of the isolated compounds were elucidated by classical spectroscopic methods including UV, NMR and HR-MS.
2012
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/574516
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact