Diospyros bipindensis (Gürke) stem barks are used in Cameroon by pygmies Baka for the treatment of pulmonary diseases. A common process in all of these diseases is inflammation. Thus, we used the inhibition of NF-κB as a target. Bioassay-guided fractionation was performed on the active dichloromethane extract. A C18 reverse solid phase column was used to fractionate the extract in 5 fractions. Active fractions 3 and 4 were further separated by semi-prep HPLC to afford 4-hydroxy-5-methylcoumarin, plumbagin, canaliculatin, ismailin and betulinic acid as the main constituents. Plumbagin and ismailin inhibited NF-κB with an IC50 of 0.9 and 29.5µM, respectively. Together with minor compounds, they could contribute to the anti-inflammatory activity of the extract. These results may support the traditional use of Diospyros bipindebsis for the treatment of pulmonary diseases.
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Titolo: | Anti-inflammatory compounds isolated from Diospyros bipindensis |
Autori: | |
Data di pubblicazione: | 2012 |
Rivista: | |
Citazione: | Anti-inflammatory compounds isolated from Diospyros bipindensis / Cesari I.; Hoerlé M.; Queiroz EF; Brusotti G; Caccialanza G; Wolfender JL; Cuendet M. - In: PLANTA MEDICA. - ISSN 0032-0943. - STAMPA. - 14(2012), pp. PI348-PI348. ((Intervento presentato al convegno ICNPR International Congress on Natural Products Research tenutosi a NY, USA nel 28/07-1/08-2012. |
Abstract: | Diospyros bipindensis (Gürke) stem barks are used in Cameroon by pygmies Baka for the treatment of pulmonary diseases. A common process in all of these diseases is inflammation. Thus, we used the inhibition of NF-κB as a target. Bioassay-guided fractionation was performed on the active dichloromethane extract. A C18 reverse solid phase column was used to fractionate the extract in 5 fractions. Active fractions 3 and 4 were further separated by semi-prep HPLC to afford 4-hydroxy-5-methylcoumarin, plumbagin, canaliculatin, ismailin and betulinic acid as the main constituents. Plumbagin and ismailin inhibited NF-κB with an IC50 of 0.9 and 29.5µM, respectively. Together with minor compounds, they could contribute to the anti-inflammatory activity of the extract. These results may support the traditional use of Diospyros bipindebsis for the treatment of pulmonary diseases. |
Handle: | http://hdl.handle.net/11571/574518 |
Appare nelle tipologie: | 4.3 Poster |