A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coli and purified. The enzyme showed a good stability and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related to compounds of biological interest via a “one pot, one enzyme” transglycosylation reaction using 7-methyl guanosine iodide as the sugar donor and 6-substituted purines as acceptor.
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Titolo: | Sintesi chemoenzimatica di nucleosidi |
Autori: | |
Data di pubblicazione: | 2012 |
Rivista: | |
Abstract: | A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coli and purified. The enzyme showed a good stability and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related to compounds of biological interest via a “one pot, one enzyme” transglycosylation reaction using 7-methyl guanosine iodide as the sugar donor and 6-substituted purines as acceptor. |
Handle: | http://hdl.handle.net/11571/582538 |
Appare nelle tipologie: | 1.1 Articolo in rivista |
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