A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coli and purified. The enzyme showed a good stability and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related to compounds of biological interest via a “one pot, one enzyme” transglycosylation reaction using 7-methyl guanosine iodide as the sugar donor and 6-substituted purines as acceptor.
Sintesi chemoenzimatica di nucleosidi
UBIALI, DANIELA;SERRA, IMMACOLATA;BORGHESE, GIOVANNI;ALBERTINI, ALESSANDRA;
2012-01-01
Abstract
A purine nucleoside phosphorylase from Aeromonas hydrophila was cloned, expressed in Escherichia coli and purified. The enzyme showed a good stability and a broad substrate specificity towards modified purine ribonucleosides. The enzyme was used for the synthesis of purine nucleosides structurally related to compounds of biological interest via a “one pot, one enzyme” transglycosylation reaction using 7-methyl guanosine iodide as the sugar donor and 6-substituted purines as acceptor.File in questo prodotto:
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