A photoreactive molecular dye targeting the G-quadruplex nucleic acid (G4) of the human telomeric sequence Tel22, and several mutated analogs, was activated by green light (λ=532 nm). Highly selective covalent modification of G4 vs. single-stranded and double-stranded DNA was achieved with efficiency up to 64 %. The phenoxyl radical was generated and detected by laser-flash photolysis as a reactive intermediate that targeted loop thymine residues. These insights may suggest a non-invasive tool for selective nucleic acid tagging and "pull-down" cellular applications.
A Photoreactive G-Quadruplex Ligand Triggered by Green Light
DORIA, FILIPPOInvestigation
;GERMANI, LUCAData Curation
;FRECCERO, MAURO
Writing – Original Draft Preparation
2015-01-01
Abstract
A photoreactive molecular dye targeting the G-quadruplex nucleic acid (G4) of the human telomeric sequence Tel22, and several mutated analogs, was activated by green light (λ=532 nm). Highly selective covalent modification of G4 vs. single-stranded and double-stranded DNA was achieved with efficiency up to 64 %. The phenoxyl radical was generated and detected by laser-flash photolysis as a reactive intermediate that targeted loop thymine residues. These insights may suggest a non-invasive tool for selective nucleic acid tagging and "pull-down" cellular applications.File in questo prodotto:
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29-07-15-Pre-Print Chem. Eur. J. 2015, 21, 2330 – 2334.pdf
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