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A photoreactive molecular dye targeting the G-​quadruplex nucleic acid (G4) of the human telomeric sequence Tel22, and several mutated analogs, was activated by green light (λ=532 nm)​. Highly selective covalent modification of G4 vs. single-​stranded and double-​stranded DNA was achieved with efficiency up to 64 %. The phenoxyl radical was generated and detected by laser-​flash photolysis as a reactive intermediate that targeted loop thymine residues. These insights may suggest a non-​invasive tool for selective nucleic acid tagging and "pull-​down" cellular applications.

A Photoreactive G-Quadruplex Ligand Triggered by Green Light

DORIA, FILIPPO
Investigation
;GERMANI, LUCA
Data Curation
;FRECCERO, MAURO
Writing – Original Draft Preparation
2015-01-01

Abstract

A photoreactive molecular dye targeting the G-​quadruplex nucleic acid (G4) of the human telomeric sequence Tel22, and several mutated analogs, was activated by green light (λ=532 nm)​. Highly selective covalent modification of G4 vs. single-​stranded and double-​stranded DNA was achieved with efficiency up to 64 %. The phenoxyl radical was generated and detected by laser-​flash photolysis as a reactive intermediate that targeted loop thymine residues. These insights may suggest a non-​invasive tool for selective nucleic acid tagging and "pull-​down" cellular applications.
2015
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CHEMISTRY-A EUROPEAN JOURNAL
sì
WOS:000348510400006
2-s2.0-84921666443
Esperti anonimi
Inglese
Internazionale
ELETTRONICO
21
21
2330
2334
5
DNA damage; G-quadruplex; Nucleic acids; photochemistry
5
info:eu-repo/semantics/article
262
Matteo, Nadai; Doria, Filippo; Germani, Luca; Sara N., Richter; Freccero, Mauro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/986085
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