The advances in proteomics and genomics have led to discover a lot of biomarkers that can potentially be used as diagnostic and prognostic indicators of diseases. Simultaneously, a huge research effort has been invested in developing biosensors that could monitor the interaction of biological materials. Such sensors are required to be fast, real time, label free and highly sensitive to the appropriate biomarker. One of the possible solutions is surface plasmon resonance imaging (iSPR) biosensor. Nevertheless, some important milestones still need to be reached for a successful application. Indeed, iSPR instruments commercially available have a poor sensitivity to low biomarker concentration and they are quite expensive. In this paper, we show a compact instrument, called Imaging NanoplasmonicsTM (iNPx), designed to overcome these limits. A nanostructured interface is introduced to increase the sensitivity of different immunoassay reactions and with the use of a very low volume of material. Then, as a proof of principle, we report an example of specific application for the monitoring of the interaction between some variants of FLAG peptides with the monoclonal antibody Anti-FLAG. The proposed platform allows extreme versatility for multiplexed diagnostic and/or food quality applications.
Nanoplasmonic platform for multiparametric and highthroughput biosensing
Carpignano, F.
;Fornasari, L.Membro del Collaboration Group
;Marabelli, F.Membro del Collaboration Group
;
2016-01-01
Abstract
The advances in proteomics and genomics have led to discover a lot of biomarkers that can potentially be used as diagnostic and prognostic indicators of diseases. Simultaneously, a huge research effort has been invested in developing biosensors that could monitor the interaction of biological materials. Such sensors are required to be fast, real time, label free and highly sensitive to the appropriate biomarker. One of the possible solutions is surface plasmon resonance imaging (iSPR) biosensor. Nevertheless, some important milestones still need to be reached for a successful application. Indeed, iSPR instruments commercially available have a poor sensitivity to low biomarker concentration and they are quite expensive. In this paper, we show a compact instrument, called Imaging NanoplasmonicsTM (iNPx), designed to overcome these limits. A nanostructured interface is introduced to increase the sensitivity of different immunoassay reactions and with the use of a very low volume of material. Then, as a proof of principle, we report an example of specific application for the monitoring of the interaction between some variants of FLAG peptides with the monoclonal antibody Anti-FLAG. The proposed platform allows extreme versatility for multiplexed diagnostic and/or food quality applications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
