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Our previous data demonstrated the presence of MSCs isolated from ovarian follicular liquid. A novel immunomagnetic procedure to isolate rere cells in suspension was used, using Dynal microbeads and a dedicated multiwells magnetic device. It was possible to isolate CD44+ cells and recovere them onto coverslips for the next steps of immunostaining and/or biological characterization. Further experiments have been designed in order to verify the stemness of these cells, seeding them in culture and inducing differentiation into other cell lineages to assess in vitro their plasticity.

Immunomagnetic separation and biological characterization of a putative mesenchymal stem cells subpopulation

RIVA, FEDERICA;OMES, CLAUDIA;ICARO CORNAGLIA, ANTONIA;CASASCO, MARCO;CASASCO, ANDREA;CALLIGARO, ALBERTO
2009-01-01

Abstract

Our previous data demonstrated the presence of MSCs isolated from ovarian follicular liquid. A novel immunomagnetic procedure to isolate rere cells in suspension was used, using Dynal microbeads and a dedicated multiwells magnetic device. It was possible to isolate CD44+ cells and recovere them onto coverslips for the next steps of immunostaining and/or biological characterization. Further experiments have been designed in order to verify the stemness of these cells, seeding them in culture and inducing differentiation into other cell lineages to assess in vitro their plasticity.
2009
Proceedings 33° National Congress of the Italian Society of Histochemistry
Cell & Developmental Biology contains resources in biochemistry, molecular biology, biophysics, physiology, and pharmacology that have a specific emphasis on cellular function in eukaryotic systems. Topics of particular importance include receptor biology and signal transduction, regulation of gene expression at the cellular level, developmental genetics, developmental biology and morphogenesis, and cell-environment interactions. Resources concentrated on molecular biochemistry and molecular regulation of gene expression, as well as microscopic or histological analysis of cell or tissue samples are excluded.
EUROPEAN JOURNAL OF HISTOCHEMISTRY
Sì, ma tipo non specificato
Irlandese Medio (900-1200)
Inglese
contributo
33° National Congress of the Italian Society of Histochemistry
8-10 June 2009
Roma
Internazionale
STAMPA
53
29
30
Human follicular liquid; stem cells; characterization
none
Riva, Federica; Omes, Claudia; Mazzini, G.; ICARO CORNAGLIA, Antonia; Casasco, Marco; Casasco, Andrea; Tinelli, C.; Calligaro, Alberto
273
info:eu-repo/semantics/conferenceObject
8
4 Contributo in Atti di Convegno (Proceeding)::4.1 Contributo in Atti di convegno
4.1 Contributo in Atti di convegno
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/149093
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