Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is commonly employed owing to its biocompatibility; in addition , the in vitro modification of hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering . In this study we have followed a biomimetic strategy where electromagnetically stimulated SAOS -2 human osteoblasts proliferated and built their extracellular matrix inside porous hydroxyapatite. In comparison with control conditions , the electromagnetic stimulus (magnetic field , 2 mT; frequency , 75 Hz) increased , in vitro, the cell proliferation and the coating of hydroxyapatite with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The physical stimulus aimed at obtaining a better in vitro modification of porous hydroxyapatite in terms of cell colonization and coating with osteogenic signals , like bone matrix proteins . The modified biomaterial could be used, in clinical applications, as an implant for bone repair.

Enhancement of a culture of human osteoblasts inside hydroxyapatite scaffolds via [2 mT; 75 Hz]-electromagnetic bioreactor

Fassina L;Saino E;Visai L;Cusella De Angelis MG;Benazzo F;Magenes G
2009-01-01

Abstract

Biomaterials have been widely used in reconstructive bone surgery to heal critical-size long bone defects due to trauma, tumor resection, and tissue degeneration. In particular, porous hydroxyapatite is commonly employed owing to its biocompatibility; in addition , the in vitro modification of hydroxyapatite with osteogenic signals enhances the tissue regeneration in vivo, suggesting that the biomaterial modification could play an important role in tissue engineering . In this study we have followed a biomimetic strategy where electromagnetically stimulated SAOS -2 human osteoblasts proliferated and built their extracellular matrix inside porous hydroxyapatite. In comparison with control conditions , the electromagnetic stimulus (magnetic field , 2 mT; frequency , 75 Hz) increased , in vitro, the cell proliferation and the coating of hydroxyapatite with bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen). The physical stimulus aimed at obtaining a better in vitro modification of porous hydroxyapatite in terms of cell colonization and coating with osteogenic signals , like bone matrix proteins . The modified biomaterial could be used, in clinical applications, as an implant for bone repair.
2009
IFMBE Proceedings
978-3-642-03900-3
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11571/244298
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