A modular and efficient nonlinear scanning microscope using an ultrashort pulse laser has been developed. The system is fully supervised by with an ad-hoc electronic system based on FPGA (Field Programmable Gate Array). A closed-loop control allows the compensation of scanning system non-idealities. Fully customizable trajectories can be used, in order to find best performances of the mechanical system. The electronic system is also characterized by the management of the target focusing on different focal planes and automatic research of the best focal plane. An ad-hoc software controls the system by a standard USB interface and processes images.
Low-cost two-photon microscope with fully customized trajectories
TOMASELLI, ALESSANDRA;VACCHI, CARLA;
2010-01-01
Abstract
A modular and efficient nonlinear scanning microscope using an ultrashort pulse laser has been developed. The system is fully supervised by with an ad-hoc electronic system based on FPGA (Field Programmable Gate Array). A closed-loop control allows the compensation of scanning system non-idealities. Fully customizable trajectories can be used, in order to find best performances of the mechanical system. The electronic system is also characterized by the management of the target focusing on different focal planes and automatic research of the best focal plane. An ad-hoc software controls the system by a standard USB interface and processes images.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
