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EnglishWe extensively employed a real-time quantitative PCR system, together with a commercially available kit (Quantifilerk Human DNA Quantification Kit, Applied Biosystems), for the quantification of human DNA in a large variety of samples. The results we obtained were reliable, with a low deviation standard for the same sample; however, when the inhibitors in a sample were in high concentration and/or DNA degradation was present (like in postmortem matrices), we observed variable results of quantification in the same extract. Here, we report our experience using this real-time based method for quantification and typing of samples recovered in forensic casework.
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| Titolo: | Quantification of human DNA by Real Time PCR in forensic casework | |
| Autori: | ||
| Data di pubblicazione: | 2006 | |
| Abstract: | We extensively employed a real-time quantitative PCR system, together with a commercially available kit (Quantifilerk Human DNA Quantification Kit, Applied Biosystems), for the quantification of human DNA in a large variety of samples. The results we obtained were reliable, with a low deviation standard for the same sample; however, when the inhibitors in a sample were in high concentration and/or DNA degradation was present (like in postmortem matrices), we observed variable results of quantification in the same extract. Here, we report our experience using this real-time based method for quantification and typing of samples recovered in forensic casework. | |
| Handle: | http://hdl.handle.net/11571/27026 | |
| ISBN: | 9780444521026 | |
| Appare nelle tipologie: | 4.1 Contributo in Atti di convegno |
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