CINECA IRIS Institutional Research Information System
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishWe extensively employed a real-time quantitative PCR system, together with a commercially available kit (Quantifilerk Human DNA Quantification Kit, Applied Biosystems), for the quantification of human DNA in a large variety of samples. The results we obtained were reliable, with a low deviation standard for the same sample; however, when the inhibitors in a sample were in high concentration and/or DNA degradation was present (like in postmortem matrices), we observed variable results of quantification in the same extract. Here, we report our experience using this real-time based method for quantification and typing of samples recovered in forensic casework.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
| Titolo: | Quantification of human DNA by Real Time PCR in forensic casework |
| Autori: | |
| Data di pubblicazione: | 2006 |
| Abstract: | We extensively employed a real-time quantitative PCR system, together with a commercially available kit (Quantifilerk Human DNA Quantification Kit, Applied Biosystems), for the quantification of human DNA in a large variety of samples. The results we obtained were reliable, with a low deviation standard for the same sample; however, when the inhibitors in a sample were in high concentration and/or DNA degradation was present (like in postmortem matrices), we observed variable results of quantification in the same extract. Here, we report our experience using this real-time based method for quantification and typing of samples recovered in forensic casework. |
| Handle: | http://hdl.handle.net/11571/27026 |
| ISBN: | 9780444521026 |
| Appare nelle tipologie: | 4.1 Contributo in Atti di convegno |
File in questo prodotto:
Copyright © 2021