Eye movements analysis represents a powerful tool in all studies dealing with visual attention and exploration, as in the case of a subject interacting with the computer display. The analysis of subject’s eye movements can provide quantitative and objective information on where the subject is looking at and for how long his/her gaze remains on a specific part of the screen. The evaluation methodology based on the analysis of exploration eye movements can be applied to any situation that implies interaction of a subject with the computer. As an example the evaluation of e-learning systems is described in this paper. The methodology based on eye movement analysis has the main characteristics to be objective and to provide quantitative results and it easily integrates the classical tests normally applied to evaluate usability.