Carrot cells contain two transcript types for the dhfr-ts gene and evidence was obtained for a plastidial localization of the product of the longer transcripts. This indication contrasted with previous circumstantial evidence which suggested a cytosolic Iodization. The conflicting evidence was found to bt due to the presence of a large intron in the region corresponding to the 5' end of the mRNA. In this paper we report the structure of the genomic region corresponding to the 5' end of the carrot DHFR-TS-encoding gene and the cloning by inverse polymerase chain reaction of the promoter region which has been partially sequenced showing the presence of putative TATA boxes and AT-rich regions. It is also observed that the two dhfr-ts gene transcripts contain TC repeats and a TC-rich stretch, respectively, whose possible role in the regulation of gene expression is discussed.

Analysis of the structure of the 5' end of the gene coding for carrot dihydrofolate reductase-thymidylate synthase.

CELLA, RINO
1995

Abstract

Carrot cells contain two transcript types for the dhfr-ts gene and evidence was obtained for a plastidial localization of the product of the longer transcripts. This indication contrasted with previous circumstantial evidence which suggested a cytosolic Iodization. The conflicting evidence was found to bt due to the presence of a large intron in the region corresponding to the 5' end of the mRNA. In this paper we report the structure of the genomic region corresponding to the 5' end of the carrot DHFR-TS-encoding gene and the cloning by inverse polymerase chain reaction of the promoter region which has been partially sequenced showing the presence of putative TATA boxes and AT-rich regions. It is also observed that the two dhfr-ts gene transcripts contain TC repeats and a TC-rich stretch, respectively, whose possible role in the regulation of gene expression is discussed.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11571/465158
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