A mini-primer set to amplify the mtDNA control region was developed and tested on fourteen long compact bone samples (femur and humerus samples) recovered from a World War II mass grave. This approach gave successful PCR amplifications for all but one of the bone samples. The following sequencing analysis identified seven different mtDNA haplotypes, three of which shared by more than one bone sample. These haplotypes were then compared to living relatives of missing persons disappeared in that area at the end of the WWII (1945).
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